Identification of Novel A2/C2 Inter-Genotype Recombinants of Hepatitis B Virus from a Korean Chronic Patient Co-Infected with Both Genotype A2 and C2open access
- Authors
- Lee, So-Young; Lee, Seung-Hee; Kim, Ji-Eun; Kim, Hong; Kim, Kijeong; Kook, Yoon-Hoh; Kim, Bum-Joon
- Issue Date
- Apr-2017
- Publisher
- MDPI AG
- Keywords
- Hepatitis B virus; polymerase RT region; small surface protein; subgenotype A2; subgenotype C2; inter-genotypical recombination
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.18, no.4
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 18
- Number
- 4
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4654
- DOI
- 10.3390/ijms18040737
- ISSN
- 1422-0067
1422-0067
- Abstract
- Nearly all cases of Hepatitis B virus (HBV) infections in South Korea have the C2 genotype. Here, we have identified a chronically infected patient who was co-infected with HBV of both the A2 and C2 genotypes by screening 135 Korean chronically infected patients using direct sequencing protocols targeting the 1032-bp polymerase reverse transcriptase (RT) region. Further polymerase chain reaction (PCR)-cloning analysis (22 clones) of the RT showed that this patient had genotype C2 (12 clones), genotype A2 (six clones) and A2/C2 inter-genotype HBV recombinants (four clones). BootScan analysis showed that three of the four recombinants have different types of recombination breakpoints in both the RT and overlapping hepatitis B surface antigen (HBsAg) region. Given the significance of HBsAg as a diagnostic or vaccination target against HBV infection, clinical implications of these identified recombinants should be studied in the future. To our knowledge, this is the first report on A2/C2 inter-genotype HBV recombinants.
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