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염증세포주를 이용한 생체재료 용출물의 체외 생체적합성 평가In vitro Biocompatibility Evaluation of Biomaterial-elution Using Inflammatory Cell Lines

Authors
신연호송계용서민지김성민박정극김동섭박기정허찬회차지훈서영권
Issue Date
2011
Publisher
한국생물공학회
Keywords
inflammation; biocompatibility; inflammatory cell line; fibroblast; cytokine
Citation
KSBB Journal, v.26, no.3, pp 248 - 254
Pages
7
Journal Title
KSBB Journal
Volume
26
Number
3
Start Page
248
End Page
254
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/46593
ISSN
1225-7117
Abstract
Various biometerials have been researched and have been developed for treatment of some disease through transplantation to body. They have been evaluated by in vitro cytotoxicity test using some skin-derived cell lines for prediction of their biocompatibility in vivo. However, the results of experiments using mesenchymal or epithelial cells could not be considered in vivo immune reaction. In this study, we evaluated the biomaterial-elution (elute from high density polyethylene film) using some cell lines (L929, Jurkat, U937) in vitro, and then that results were compared with in vivo results from guinea pig sensitization test. In sensitization test, saline and elution of syringe could not induce erythema, but only DNCB (hypersensitive chemical) induce erythema at guinea pig sensitization test. In cell experiment, the cytotoxicity results of inflammatory cells (Jurkat; T lymphocyte, U937; monocyte) was no difference with L929 (fibroblast) in the overall trend. However,inflammatory cell lines were only secreted inflammatory cytokine (TNF-α, INF-γ) in some materials (biomateriallution,FAC, DNCB). And the biomaterial-elution did not have toxicity to the cells, but it induced the inflammatory cytokines in inflammatory cell lines only. So, we were predicted inflammatory reaction through the cytokine resultes of inflammatory cell lines, and it was more correlated with in vivo results than cytotoxicity test. Therefore, we suggested that the inflammatory cytokine assay using inflammatory cell lines are more effective method in vitro for evaluation of biocompatibility of biomaterials or chemicals.
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