WNT/beta-catenin pathway modulates the TNF-alpha-induced inflammatory response in bronchial epithelial cells
- Authors
- Jang, Jaewoong; Jung, Yoonju; Chae, Seyeon; Chung, Sang-In; Kim, Seok-Min; Yoon, Yoosik
- Issue Date
- Mar-2017
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- TNF-alpha; WNT; beta-catenin; Inflammation; Bronchial epithelial cell
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.484, no.2, pp 442 - 449
- Pages
- 8
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 484
- Number
- 2
- Start Page
- 442
- End Page
- 449
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4695
- DOI
- 10.1016/j.bbrc.2017.01.156
- ISSN
- 0006-291X
1090-2104
- Abstract
- In this study, TNF-alpha was found to activate the WNT/beta-catenin pathway in BEAS-2B human bronchial epithelial cells. Levels of phospho-LRP6, Dv1-2, and phospho-GSK-3 beta were elevated, while that of Axin was reduced by TNF-alpha treatment. Nuclear translocation of beta-catenin and the reporter activity of a beta-catenin-responsive promoter were increased by TNF-alpha treatment. Under the same experimental conditions, TNF-alpha activated the NF-kappa B signaling, which includes the phosphorylation and degradation of I kappa B and nuclear translocation and target DNA binding of NF-kappa B, and it was found that an inhibitor of NF-kappa B activation, JSH-23, inhibited TNF-alpha-induced Wnt signaling as well as NF-kappa B signaling. It was also found that recombinant Wnt proteins induced NF-kappa B nuclear translocations and its target DNA binding, suggesting that Wnt signaling and NF-kappa B signaling were inter-connected. TNF-alpha-induced modulations of I kappa B and NE-kappa B as well as pro-inflammatory cytokine expression were significantly suppressed by the transfection of beta-catenin siRNA compared to that of control siRNA. Transfection of a beta-catenin expression plasmid augmented the TNF-alpha-induced modulations of I kappa B and NF-kappa B as well as pro-inflammatory cytokine expression. These results clearly demonstrated that the WNT/beta-catenin pathway modulates the inflammatory response induced by TNF-alpha, suggesting that this pathway may be a useful target for the effective treatment of bronchial inflammation. (C) 2017 Published by Elsevier Inc.
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Collections - College of Engineering > School of Mechanical Engineering > 1. Journal Articles
- College of Medicine > College of Medicine > 1. Journal Articles
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