Prevalence of hepatitis E virus antibodies in Canadian swine herds and identification of a novel variant of swine hepatitis E virusopen access
- Authors
- Yoo, D; Willson, P; Pei, YL; Hayes, MA; Deckert, A; Dewey, CE; Friendship, RM; Yoon, YH; Gottschalk, M; Yason, C; Giulivi, A
- Issue Date
- Nov-2001
- Publisher
- AMER SOC MICROBIOLOGY
- Citation
- CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, v.8, no.6, pp 1213 - 1219
- Pages
- 7
- Journal Title
- CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
- Volume
- 8
- Number
- 6
- Start Page
- 1213
- End Page
- 1219
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47224
- DOI
- 10.1128/CDLI.8.6.1213-1219.2001
- ISSN
- 1071-412X
- Abstract
- Swine hepatitis E virus is a newly identified potentially zoonotic virus from pigs of particular concern for possible direct transmission to a human xenotransplant recipient by organ transplantation. In the present study, prevalence of serum antibodies to hepatitis E virus was examined in Canadian swine herds. A total of 998 serum samples collected from 6-month-old healthy slaughter hogs were examined by enzyme immunoassay and Western blot analysis for antibodies to the recombinant open reading frame 3 (ORF3) protein of hepatitis E virus expressed in Escherichia coli. These samples represented more than 80 different swine production units from five major swine-producing provinces across Canada. From this study, 594 samples (59.4%) were found to be positive for hepatitis E virus antibody. The seroprevalence was higher in Quebec (88.8%) and Ontario (80.1%) than in Alberta and Saskatchewan (38.3%). By PCR using a pair of oligonucleotide primers deduced from the ORF2 sequence of human hepatitis E virus, a specific hepatitis E virus sequence was recovered from feces of pigs. The nucleotide sequence identity between the U.S. swine hepatitis E virus and the Canadian isolate (SK3) was only 85.8%, suggesting that genotypic variations may exist in swine hepatitis E virus in North America. Among 165 serum samples collected from humans in Saskatchewan, 2.4% were found to be positive for antibodies to the hepatitis E virus ORF3 protein. Our data indicate that hepatitis E virus is highly prevalent in commercial swine populations in Canada and support the suggestion that the swine hepatitis E virus may be an important zoonotic agent for humans.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47224)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.