Involvement of caspase-3 in apoptosis induced by Viscum album var. coloratum agglutinin in HL-60 cells
- Authors
- Lyu, SY; Park, WB; Choi, KH; Kim, WH
- Issue Date
- Mar-2001
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Korean mistletoe; lectin; apoptosis; caspase-3; poly(ADP-ribose)polymerase
- Citation
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.65, no.3, pp 534 - 541
- Pages
- 8
- Journal Title
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Volume
- 65
- Number
- 3
- Start Page
- 534
- End Page
- 541
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47271
- DOI
- 10.1271/bbb.65.534
- ISSN
- 0916-8451
1347-6947
- Abstract
- A cytotoxic lectin (Viscum album L. coloratum agglutinin, VCA) from Korean mistletoe was isolated by affinity chromatography on Sepharose 4B immobilized with asialofetuin. In HL-60 cells, addition of VCA resulted in a dose- and time-dependent growth suppression, morphological changes of apoptotic nuclei, and DNA fragmentation characteristics of apoptosis, To investigate how caspase-3 activation during VCA-induced apoptosis induces cleavages of PARP, the expression of PARP and the pattern of caspase-3 activation in HL-60 cells were investigated. The native and processed PARP forms typically seen in apoptotic cells were observed, and a decrease in expression of the 32-kDa form of caspase-3 in a dose-dependent manner was observed. The VCA-induced apoptosis was significantly inhibited by a caspase-3 specific inhibitor, z-DEVD-FMK, and the PARP processing and caspase-3 activation were also inhibited by the inhibitor. A possible involvement of cell cycle arrest in VCA-induced apoptosis was investigated by flow cytometry and the results suggested that the apoptotic effect of VCA is not involved in the induction of cell cycle arrest.
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