A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis
- Authors
- Chung, JY; Bahk, YY; Huh, S; Kang, SY; Kong, Y; Cho, SY
- Issue Date
- Oct-1999
- Publisher
- UNIV CHICAGO PRESS
- Citation
- JOURNAL OF INFECTIOUS DISEASES, v.180, no.4, pp 1307 - 1315
- Pages
- 9
- Journal Title
- JOURNAL OF INFECTIOUS DISEASES
- Volume
- 180
- Number
- 4
- Start Page
- 1307
- End Page
- 1315
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47420
- DOI
- 10.1086/315020
- ISSN
- 0022-1899
1537-6613
- Abstract
- Neurocysticercosis (NCC) is an important cause of neurological disease worldwide. A 10-kDa antigen of Taenia solium metacestodes (TsMs) has been shown to be specific for immunodiagnosis of NCC, Screening of a TsM complementary DNA (cDNA) library isolated a cDNA encoding this protein. The cloned cDNA contained a 258-bp complete open-reading frame that encodes an 86-amino acid polypeptide with a calculated molecular weight of 9582 Da. It showed 73% homology with a 10-kDa antigen of T. crassiceps. The recombinant protein was expressed bacterially as a fusion protein at a high level. In immunoblot with recombinant protein, 97% (184/190) of sera from patients with active NCC showed strong reactivity, whereas 14% (4/29) of those from patients with chronic calcified NCC reacted weakly. In 180 sera from other patients with parasitic infections and from normal controls, it showed 98% specificity A single recombinant TsM antigen has a high potential for serological differentiation of active NCC.
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