Characterization of Prototype Foamy Virus Infectivity in Transportin 3 Knockdown Human 293t Cell Line
- Authors
- Bin Hamid, Faysal; Kim, Jinsun; Shin, Cha-Gyun
- Issue Date
- Feb-2017
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Transportin-3; prototype foamy virus; 1-long terminal repeat; 293 T cell
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.27, no.2, pp 380 - 387
- Pages
- 8
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 27
- Number
- 2
- Start Page
- 380
- End Page
- 387
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4852
- DOI
- 10.4014/jmb.1606.06011
- ISSN
- 1017-7825
1738-8872
- Abstract
- The foamy viruses are currently considered essential for development as vectors for gene delivery. Previous studies demonstrated that prototype foamy virus (PFV) can infect and replicate prevalently in a variety of cell types for its exclusive replication strategy. However, the virus-host interaction, especially PFV-transportin3 (TNPO3), is still poorly understood. In our investigation of the role of TNPO3 in PFV infection, we found lower virus production in TNPO3 knockdown (KD) cells compared with wild-type 293T cells. PCR analysis revealed that viral DNAs were mostly altered to circular forms: both 1-long terminal repeat (1-LTR) and 2-LTR in TNPO3 KD cells. We therefore suggest that TNPO3 is required for successful PFV replication, at least at/after the nuclear entry step of viral DNA. These findings highlight the obscure mysteries of PFV-host interaction and the requirement of TNPO3 for productive infection of PFV in 293T cells.
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