EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells
- Authors
- Jin, Hanyong; Won, Miae; Shin, Eunkyoung; Kim, Hong-Man; Lee, Kangseok; Bae, Jeehyeon
- Issue Date
- Jan-2017
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- KROX20; FSH; LH; Transcriptional regulation; Target gene
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.482, no.4, pp 877 - 882
- Pages
- 6
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 482
- Number
- 4
- Start Page
- 877
- End Page
- 882
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4903
- DOI
- 10.1016/j.bbrc.2016.11.127
- ISSN
- 0006-291X
1090-2104
- Abstract
- Pituitary gonadotropins are key hormones that orchestrate the growth and development of ovarian follicles. However, limited information is available on intra-ovarian factors that mediate the actions of gonadotropins. In this study, we identified that the early growth response 2 gene (EGR2) is a gonadotropin-inducible gene in granulosa cells of rats and humans. Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. Overexpression of EGR2 promoted survival of KGN human granulosa-derived cells in which IER3 acts as a mediator; knockdown of EGR2 induced death in KGN cells. Additionally, EGR2 was found to regulate the expression of myeloid cell leukemia 1 (MCL-1), which belongs to the BCL-2 family of proteins regulating cell survival. Thus, this study identified a novel signaling axis, comprised of gonadotropins-EGR2-IER3, which is important for the survival of granulosa cells during folliculogenesis. (C) 2016 Elsevier Inc. All rights reserved.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
- College of Natural Sciences > Department of Life Science > 1. Journal Articles
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