A method to monitor dutasteride in rat plasma using liquid-liquid extraction and multiple reaction monitoring: Comparisons and validation
- Authors
- Kang, M.J.; Cho, H.R.; Lee, D.H.; Yeom, D.W.; Choi, Y.W.; Choi, Y.S.
- Issue Date
- 1-Sep-2014
- Publisher
- Korean Society for Mass Spectrometry
- Keywords
- Dutasteride; Multiple reaction monitoring; Rat plasma
- Citation
- Mass Spectrometry Letters, v.5, no.3, pp 79 - 83
- Pages
- 5
- Journal Title
- Mass Spectrometry Letters
- Volume
- 5
- Number
- 3
- Start Page
- 79
- End Page
- 83
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50050
- DOI
- 10.5478/MSL.2014.5.3.79
- ISSN
- 2233-4203
2093-8950
- Abstract
- Three different dutasteride extraction methods were compared and a method based on liquid-liquid extraction (LLE) using methyl tert-butyl ether and methylene chloride was proved to be more effective than others for the extraction of dutasteride and finasteride, the internal standard (IS), from rat plasma. Additionally, a method composed of the LLE extraction, liquid chromatography, and multiple reaction monitoring (MRM) to target dutasteride and IS was validated by assessing specificity, linearity (r2 = 0.9993, 5 - 400 ng/mL), sensitivity (the limit of detection: 4.03 ng/mL; the limit of quantitation: 12.10 ng/mL), accuracy (intra-day: 89.4 - 105.9%; inter-day: 84.9 - 100.9%), precision (intra-day: 0.8 - 6.9%; inter-day: 2.9 - 15.9%), and recovery (84.7 - 107.8%). Since the validated method was successfully applied to a pharmacokinetic study of dutasteride, it can be useful for the pharmacokinetic evaluation of newly developed dutasteride formulations. © 2014, Korean Society for Mass Spectrometry. All right reserved.
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