Improving osteogenesis activity on BMP-2-immobilized PCL fibers modified by the γ -ray irradiation technique
- Authors
- Yun, Y.-P.; Lee, J.Y.; Jeong, W.J.; Park, K.; Kim, H.-J.; Song, J.-J.; Kim, S.E.; Song, H.-R.
- Issue Date
- 2015
- Publisher
- Hindawi Publishing Corporation
- Citation
- BioMed Research International, v.2015
- Journal Title
- BioMed Research International
- Volume
- 2015
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50229
- DOI
- 10.1155/2015/302820
- ISSN
- 2314-6133
2314-6141
- Abstract
- The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the γ-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the γ-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP-2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and the mRNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells. © 2015 Young-Pil Yun et al.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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