A quartz nanopillar hemocytometer for high-yield separation and counting of CD4(+) T lymphocytes
- Authors
- Kim, Dong-Joo; Seol, Jin-Kyeong; Wu, Yu; Ji, Seungmuk; Kim, Gil-Sung; Hyung, Jung-Hwan; Lee, Seung-Yong; Lim, Hyuneui; Fan, Rong; Lee, Sang-Kwon
- Issue Date
- 2012
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- NANOSCALE, v.4, no.7, pp 2500 - 2507
- Pages
- 8
- Journal Title
- NANOSCALE
- Volume
- 4
- Number
- 7
- Start Page
- 2500
- End Page
- 2507
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50374
- DOI
- 10.1039/c2nr11338d
- ISSN
- 2040-3364
2040-3372
- Abstract
- We report the development of a novel quartz nanopillar (QNP) array cell separation system capable of selectively capturing and isolating a single cell population including primary CD4(+) T lymphocytes from the whole pool of splenocytes. Integrated with a photolithographically patterned hemocytometer structure, the streptavidin (STR)-functionalized-QNP (STR-QNP) arrays allow for direct quantitation of captured cells using high content imaging. This technology exhibits an excellent separation yield (efficiency) of similar to 95.3 +/- 1.1% for the CD4(+) T lymphocytes from the mouse splenocyte suspensions and good linear response for quantitating captured CD4(+) T-lymphoblasts, which is comparable to flow cytometry and outperforms any non-nanostructured surface capture techniques, i.e. cell panning. This nanopillar hemocytometer represents a simple, yet efficient cell capture and counting technology and may find immediate applications for diagnosis and immune monitoring in the point-of-care setting.
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Collections - College of Natural Sciences > Department of Physics > 1. Journal Articles
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