Heparin/poly(L-lysine) nanoparticle-coated polymeric microspheres for stem-cell therapy
- Authors
- Na, Kun; Kim, Sungwon; Park, Kyeongsoon; Kim, Kwangmeyung; Woo, Dae Gyun; Kwon, Ick Chan; Chung, Hyung-Min; Park, Keun-Hong
- Issue Date
- May-2007
- Publisher
- AMER CHEMICAL SOC
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.129, no.18, pp 5788 - +
- Journal Title
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- Volume
- 129
- Number
- 18
- Start Page
- 5788
- End Page
- +
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51432
- DOI
- 10.1021/ja067707r
- ISSN
- 0002-7863
1520-5126
- Abstract
- Three-dimensional microspheres have been used extensively in several biomaterials fields, in applications such as tissue-regeneration scaffolds and drug delivery systems. To apply these biomaterials as novel cell therapeutic agents, we have devised a novel method for the fabrication of nanostructured 3D scaffolds consisting of heparin/poly(l-lysine) nanoparticles on the surface of polymeric microspheres, attached via a layer-by-layer (LbL) system. The initial step of this strategy involves the creation of heparin/poly(l-lysine) nanoparticles, which were simply produced as polyion complex micelles (PICM) with diameters of 200-500 nm. In the second step, the heparin/poly(l-lysine) nanoparticles were coated onto positively charged poly(lactic-co-glycolic acid) (PLGA) pretreated with polyethyleneimine (PEI). The production of the heparin/poly(l-lysine) nanoparticles and their subsequent coating onto PLGA microspheres represents a novel method for the functionalization of the polymeric matrix, which requires the cellular active surfaces of nanoscaled heparinized surfaces as 3D scaffolds, thus creating a better microenvironment of cell adhesion and growth for use in cell therapy applications.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51432)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.