High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis
- Authors
- Song, Hana; Lee, Sun-Young
- Issue Date
- Jun-2021
- Publisher
- WILEY
- Keywords
- Escherichia coli O157; H7; NaCl; pH; Salmonella enterica serovar Enteritidis; survival; VBNC
- Citation
- LETTERS IN APPLIED MICROBIOLOGY, v.72, no.6, pp 741 - 749
- Pages
- 9
- Journal Title
- LETTERS IN APPLIED MICROBIOLOGY
- Volume
- 72
- Number
- 6
- Start Page
- 741
- End Page
- 749
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51917
- DOI
- 10.1111/lam.13468
- ISSN
- 0266-8254
1472-765X
- Abstract
- In the present study, Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis were transferred into Luria-Bertani medium without NaCl (LBWS) and adjusted to various pHs (4, 5, 6 and 7) with lactic acid containing 0 center dot 75, 5, 10 and 30% NaCl, and stored at 25 degrees C until the bacterial populations reached below detectable levels on tryptic soy agar (TSA). Although E. coli O157:H7 and S. Enteritidis did not grow on TSA when incubated in LBWS with 30% NaCl for 35 and 7 days, more than 60 and 70% of the bacterial cells were shown to be viable via fluorescent staining with SYTO9 and propidium iodide (PI), respectively, suggesting that a number of cells could be induced into the viable but nonculturable (VBNC) state. These bacteria that were induced into a VBNC state were transferred to a newly prepared tryptic soy broth (TSB) and then incubated at 37 degrees C for several days. After more than 7 days, E. coli O157:H7 and S. Enteritidis regained their culturability. We, therefore, suggest that E. coli O157:H7 and S. Enteritidis entered the VBNC state under the adverse condition of higher salt concentrations and were revived when these conditions were reversed.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > School of Food Science and Technology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51917)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.