Evaluating the Engineered Saccharomyces cerevisiae With High Spermidine Contents for Increased Tolerance to Lactic, Succinic, and Malic Acids and Increased Xylose Fermentation

Abstract

Saccharomyces cerevisiae is a promising candidate for production of organic acids as it is more tolerant to these acids than the prokaryotes. However, the large-scale production of organic acids from lignocellulosic biomass is limited by their accumulation in the growth medium and inability of xylose fermentation by S. cerevisiae. Here we showed that high intracellular spermidine (SPD) contents confers enhanced tolerance to lactic, succinic, and malic acids in S. cerevisiae. Specifically, in the presence of 20 g/L malic acid, the maximum specific growth rate and dry cell weight of a S. cerevisiae with two fold higher SPD content were 40% and 36% higher than those of the control strain. When a xylose assimilation pathway was introduced into an engineered strain with high SPD content, the resulting S. cerevisiae strain exhibited 23 similar to 47% higher xylose consumption rate and 6 similar to 16% higher ethanol productivity than those of the control strain during the four times of repeated-batch fermentations using a mixture of glucose and xylose as carbon sources. These results suggest that the strain developed in this study would serve as a platform strain for production of organic acids.