Hyperthermal paclitaxel-bound albumin nanoparticles co-loaded with indocyanine green and hyaluronidase for treating pancreatic cancersHyperthermal paclitaxel-bound albumin nanoparticles co-loaded with indocyanine green and hyaluronidase for treating pancreatic cancers
- Authors
- Kim, Sung Soo; Kim, Hwang Kyung; Kim, Hanju; Lee, Woo Tak; Lee, Eun Seong; Oh, Kyung Taek; Choi, Han-Gon; Youn, Yu Seok
- Issue Date
- Feb-2021
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Indocyanine green; Hyaluronidase; Hyperthermal therapy; Albumin nanoparticles; Tumor targeting
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.44, no.2, pp 182 - 193
- Pages
- 12
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 44
- Number
- 2
- Start Page
- 182
- End Page
- 193
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/52674
- DOI
- 10.1007/s12272-020-01264-9
- ISSN
- 0253-6269
1976-3786
- Abstract
- Albumin nanoparticles have become an attractive cancer nanomedicine platform due to their pharmaceutical advantages. Recently, photothermal therapy has been extensively applied to cancer treatment due to heat-induced tumor ablation. Herein, we fabricated albumin nanoparticles (HSA-NPs) loaded with paclitaxel (PTX), indocyanine green (ICG; a hyperthermal agent) and hyaluronidase (HAase) that breaks down hyaluronan, a major component of the extracellular matrix (ECM) in tumors. Synthesis was based on a slightly modified nanoparticle albumin-bound (Nab (TM)) technique. The prepared nanoparticles (PTX/ICG/HAase-HSA-NPs) had a spherical shape with an average size of similar to 110 nm and a zeta potential of similar to -30.4 mV. They displayed good colloidal stability and typical patterns of ICG, HSA and HAase in UV-VIS-NIR and circular dichroism spectroscopic analysis. PTX/ICG/HAase-HSA-NPs were found to have excellent hyperthermal effects in response to near-infrared laser irradiation (808 nm) (up to > 50 degrees C over 4 min). The hyperthermia conducted by PTX/ICG/HAase-HSA-NPs resulted in significant cytotoxicity to pancreatic AsPC-1 cells at both severe (> 50 degrees C) and mild (41-42 degrees C) hyperthermal states in conjunction with the inherent cytotoxic activity of paclitaxel. Furthermore, the confocal images of AsPC-1 cell spheroids proved PTX/ICG/HAase-HSA-NPs were able to permeate deeply into the three-dimensional tumor tissue mimicry structure. Most of all, PTX/ICG/HAase-HSA-NPs maintained all these physicochemical and anti-cancer properties irrespective of the amount of embedded HAase (1-5 mg). Our results demonstrated that PTX/ICG/HAase-HSA-NPs are a promising hyperthermal/chemotherapeutic anticancer agent.
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