Facile and foldable point-of-care biochip for nucleic acid based-colorimetric detection of murine norovirus in fecal samples using G-quadruplex and graphene oxide coated microbeads
- Authors
- Cui, W.Y.; Yoo, H.J.; Li, Y.G.; Baek, C.; Min, J.
- Issue Date
- Mar-2022
- Publisher
- Elsevier Ltd
- Keywords
- Fecal samples; Graphene oxide-coated microbeads; Murine norovirus; Nucleic-acid based detection; On-site biosensor; Sample preparation
- Citation
- Biosensors and Bioelectronics, v.199
- Journal Title
- Biosensors and Bioelectronics
- Volume
- 199
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/52810
- DOI
- 10.1016/j.bios.2021.113878
- ISSN
- 0956-5663
1873-4235
- Abstract
- Norovirus is one of the most common causes of gastroenteritis, a disease characterized by diarrhea, vomiting, and stomach pain. A rapid on-site identification of the virus from fecal samples of patients is a prerequisite for accurate medical management. Here, we demonstrate a rapid nucleic acid-based detection platform as an on-site biosensing tool that can concentrate viruses from fecal samples. Moreover, it can perform RNA extraction and identification, and signal amplification using G-quadruplex and hemin containing DNA probes (G-DNA probes) and graphene oxide (GO)-coated microbeads. Briefly, murine noroviruses are lysed without chemicals on the surface of the GO microbeads. Subsequently, the target RNA is hybridized with G-DNA probes, and the resultant RNA/G-DNA probe complex is separated from unbound G-DNA probes using GO beads and is mixed with the detection buffer (ABTS/H2O2). Presence of murine noroviruses causes a colorimetric change of the buffer from colorless to green. Thus, we integrated all processes required to detect murine noroviruses in stool samples in a simple foldable microfluidic chip. Moreover, it can detect 101 pfu of the virus in 30 min in a fecal sample. © 2021 Elsevier B.V.
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