Photoinduced cleavage by a rhodium complex at G center dot U mismatches and exposed guanines in large and small RNAs
- Authors
- Chow, Christine S.; Cunningham, Philip R.; Lee, Kangseok; Meroueh, May; SantaLucia, John; Varma, Shikha
- Issue Date
- Sep-2002
- Publisher
- ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
- Keywords
- rhodium; hairpin; cleavage; chemical probing; 16S rRNA
- Citation
- BIOCHIMIE, v.84, no.9, pp 859 - 868
- Pages
- 10
- Journal Title
- BIOCHIMIE
- Volume
- 84
- Number
- 9
- Start Page
- 859
- End Page
- 868
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53432
- DOI
- 10.1016/S0300-9084(02)01403-7
- ISSN
- 0300-9084
1638-6183
- Abstract
- Photoinduced cleavage reactions by the rhodium complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) {Rh(DIP)(3)(3+)} with three RNA hairpins, r(GGGGU UCGCUC CACCA) (16,nucleotide, tetraloop(Al2)), r(GGGGCUAUAGCUCUAGCUC CACCA) (24 nucleotide, microhelix(Ala)), and r(GGCGGUUAGAUAUCGCC) (17 nucleotide, 790 loop), and full-length (1542 nucleotide) 16S rRNA from Escherichia coli were investigated. The cleavage reactions were monitored by gel electrophoresis and the sites of cleavage by Rh(DIP)(3)(3+) were determined by comparisons with chemical or enzymatic sequencing reactions. In general, RNA backbone scission by the metal complex was induced at G-U mismatches and at exposed G residues. The cleavage activity was observed on the three small RNA hairpins as well as on the isolated 1542-nucleotide ribosomal RNA. (C) 2002 Societe francaise de biochimie et biologie moleculaire/Editions scientifiques et medicales Elsevier SAS. All rights reserved.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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