Anti-inflammatory effect of neo-lignan isoamericanin A via suppression of NF-kappa B in liposaccharide-stimulated RAW 264.7 cells
- Authors
- Yeo, Ji Yun; Hwang, Kwang Woo; Park, So-Young
- Issue Date
- Sep-2020
- Publisher
- PHARMACOTHERAPY GROUP
- Keywords
- Opuntia humifusa seeds; Isoamericanin A; Nitric oxide; RAW 264.7 cells; NF-kappa B
- Citation
- TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, v.19, no.9, pp 1857 - 1862
- Pages
- 6
- Journal Title
- TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
- Volume
- 19
- Number
- 9
- Start Page
- 1857
- End Page
- 1862
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53703
- DOI
- 10.4314/tjpr.v19i9.9
- ISSN
- 1596-5996
1596-9827
- Abstract
- Purpose: To investigate the potential anti-inflammatory effects of the seeds of Opuntina humifusa and its active constituents. Methods: The extract of O. humifusa seeds was tested for the inhibition of nitric oxide (NO) production in liposaccharide (LPS)-stimulated RAW 264.7 cells using Griess reagent. The active constituents were isolated using bioassay-guided isolation methods. The effects of the active constituent on NO, proinflammatory cytokines, nuclear factor-kappa B (NF-kappa B) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (I kappa B) were evaluated by enzyme-linked immunosorbent assay (ELISA) and western blot analysis. Results: The seed extract of O. humifusa significantly attenuated LPS-induced NO production in RAW 264.7 cells (p < 0.05). Bioassay-guided fractionation resulted in the isolation of isoamericanin A as an active constituent. Isoamericanin A reduced LPS-induced production of NO, iNOS, and proinflammatory cytokines (TNF-alpha and IL-6) in a concentration-dependent manner (p < 0.05). Furthermore, the effect was accompanied by decreased translocation of NF-kappa B from the cytosol to the nucleus and the decreased phosphorylation of I kappa B in the cytosol induced by LPS (p < 0.05). Conclusion: The seed extract of O. humifusa and its active constituent, isoamericanin A, have antiinflammatory effects in LPS-stimulated RAW 264.7 cells, suggesting that they have potentials as antiinflammatory agents.
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