PLGA microsphere construct coated with TGF-beta 3 loaded nanoparticles for neocartilage formation
- Authors
- Park, Ji Sun; Park, Kyeongsoon; Woo, Dae Gyun; Yang, Han Na; Chung, Hyung-Min; Park, Keun-Hong
- Issue Date
- Aug-2008
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOMACROMOLECULES, v.9, no.8, pp 2162 - 2169
- Pages
- 8
- Journal Title
- BIOMACROMOLECULES
- Volume
- 9
- Number
- 8
- Start Page
- 2162
- End Page
- 2169
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/54298
- DOI
- 10.1021/bm800251x
- ISSN
- 1525-7797
1526-4602
- Abstract
- Polymeric microsphere system has been widely used in tissue-regeneration matrix and drug delivery systems. To apply these biomaterials as novel cell supporting matrix for stem cell delivery, we have devised a novel method for the fabrication of nanostructured 3D scaffolds that growth factor loaded heparin/poly(L-lysine) nanoparticles were physically attached on the positively charged surface of PLGA microspheres precoated with low molecular weight of poly(ethyleneimmine) (PEI) via a layer-by-layer (LbL) system. Based on a previous study, we have prepared poly(lactide-co-glycolide) (PLGA) microspheres harboring heparin/poly(L-lysine) loaded with growth factors. Growth factor loaded heparin/poly(L-lysine) nanoparticles, which were simply produced as polyion complex micelles (PICM) with diameters of 50-150 nm, were fabricated in the first step. Microsphere matrix (size, 20 similar to 80 nm) containing TGF-beta 3 showed a significantly higher number of specific lacunae phenotypes at the end of the 4 week study in vitro culture of mesenchymal stem cells. Thus, growth factor delivery of PLGA microsphere can be used to engineer synthetic extracellular matrix. This PLGA microsphere matrix containing TGF-beta 3 showed promise as coatings for implantable biomedical devices to improve biocompatibility and ensure in vivo performance.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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