Phospholipid composition analysis of krill oil through HPLC with ELSD: Development, validation, and comparison with 31P NMR spectroscopy
DC Field | Value | Language |
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dc.contributor.author | Yu, Hyo-Yeon | - |
dc.contributor.author | Park, Se-Eun | - |
dc.contributor.author | Chun, Hyang Sook | - |
dc.contributor.author | Rho, Jung-Rae | - |
dc.contributor.author | Ahn, Sangdoo | - |
dc.date.accessioned | 2022-02-10T03:44:20Z | - |
dc.date.available | 2022-02-10T03:44:20Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 0889-1575 | - |
dc.identifier.issn | 1096-0481 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/54934 | - |
dc.description.abstract | A method wherein a high-performance liquid chromatography (HPLC) system is combined with an evaporative light scattering detector (ELSD) was developed and validated as an alternative to the official phosphorous-31 nuclear magnetic resonance (31P NMR) technique to analyze phospholipids in krill oil. A quadratic calibration curve with a good correlation coefficient (>0.999) in the 100–1000 μg mL−1 range was obtained for each phospholipid. The limit of detection (2–7.5 μg mL−1) of the designed assay for the phospholipids in krill oil was good, and the accuracy (99.6–101.0 %) was excellent. The relative standard deviations for repeatability (<1%) and intermediate precision (2.3 %–6.9 %) were acceptable. The results of the developed HPLC-ELSD method applied to krill oil samples were in agreement with those of 31P NMR spectroscopy. Moreover, statistical comparison indicated no significant difference between two methods. Thus, the HPLC-ELSD method can be applied for analyzing the phospholipid composition of krill oil. © 2022 Elsevier Inc. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press Inc. | - |
dc.title | Phospholipid composition analysis of krill oil through HPLC with ELSD: Development, validation, and comparison with 31P NMR spectroscopy | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jfca.2022.104408 | - |
dc.identifier.bibliographicCitation | Journal of Food Composition and Analysis, v.107 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000791660500011 | - |
dc.identifier.scopusid | 2-s2.0-85123096982 | - |
dc.citation.title | Journal of Food Composition and Analysis | - |
dc.citation.volume | 107 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | 31P NMR spectroscopy | - |
dc.subject.keywordAuthor | Evaporative light scattering detector | - |
dc.subject.keywordAuthor | HPLC | - |
dc.subject.keywordAuthor | Krill oil | - |
dc.subject.keywordAuthor | Method validation | - |
dc.subject.keywordAuthor | Phospholipid | - |
dc.subject.keywordPlus | EVAPORATIVE LIGHT-SCATTERING | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | DAIRY-PRODUCTS | - |
dc.subject.keywordPlus | INFANT FORMULA | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | MILK | - |
dc.subject.keywordPlus | SEPARATIONS | - |
dc.subject.keywordPlus | DETECTOR | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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