Phospholipid composition analysis of krill oil through HPLC with ELSD: Development, validation, and comparison with 31P NMR spectroscopy
- Authors
- Yu, Hyo-Yeon; Park, Se-Eun; Chun, Hyang Sook; Rho, Jung-Rae; Ahn, Sangdoo
- Issue Date
- Apr-2022
- Publisher
- Academic Press Inc.
- Keywords
- 31P NMR spectroscopy; Evaporative light scattering detector; HPLC; Krill oil; Method validation; Phospholipid
- Citation
- Journal of Food Composition and Analysis, v.107
- Journal Title
- Journal of Food Composition and Analysis
- Volume
- 107
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/54934
- DOI
- 10.1016/j.jfca.2022.104408
- ISSN
- 0889-1575
1096-0481
- Abstract
- A method wherein a high-performance liquid chromatography (HPLC) system is combined with an evaporative light scattering detector (ELSD) was developed and validated as an alternative to the official phosphorous-31 nuclear magnetic resonance (31P NMR) technique to analyze phospholipids in krill oil. A quadratic calibration curve with a good correlation coefficient (>0.999) in the 100–1000 μg mL−1 range was obtained for each phospholipid. The limit of detection (2–7.5 μg mL−1) of the designed assay for the phospholipids in krill oil was good, and the accuracy (99.6–101.0 %) was excellent. The relative standard deviations for repeatability (<1%) and intermediate precision (2.3 %–6.9 %) were acceptable. The results of the developed HPLC-ELSD method applied to krill oil samples were in agreement with those of 31P NMR spectroscopy. Moreover, statistical comparison indicated no significant difference between two methods. Thus, the HPLC-ELSD method can be applied for analyzing the phospholipid composition of krill oil. © 2022 Elsevier Inc.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/54934)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.