Identification of the genome sequence of Zostera associated varicosavirus 1, a novel negative-sense RNA virus, in the common eelgrass (Zostera marina) transcriptomeopen access
- Authors
- Shin, Chaerim; Choi, Dongjin; Hahn, Yoonsoo
- Issue Date
- Aug-2021
- Publisher
- AEPRESS SRO
- Keywords
- Zostera associated varicosavirus 1; Varicosavirus; Rhabdoviridae; common eelgrass; Zostera marina
- Citation
- ACTA VIROLOGICA, v.65, no.4, pp 373 - 380
- Pages
- 8
- Journal Title
- ACTA VIROLOGICA
- Volume
- 65
- Number
- 4
- Start Page
- 373
- End Page
- 380
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55090
- DOI
- 10.4149/av_2021_404
- ISSN
- 0001-723X
1336-2305
- Abstract
- Varicosaviruses (the genus Varicosavirus) are bipartite, negative-sense, single-stranded RNA viruses that infect plants. We analyzed a transcriptome dataset isolated from the common eelgrass (Zostera marina) and identified a novel varicosavirus named Zostera associated varicosavirus 1 (ZaVV1). The ZaVV1 genome consists of two genomic segments: RNA1 (6,632-nt) has an open reading frame (ORF) encoding a large multi-functional polymerase protein (L), while RNA2 (4,304-nt) has four ORFs: one for a nucleocapsid protein and three for proteins with unknown functions (P2, P3, and P4). Sequence comparison and phylogenetic analysis using L proteins showed that ZaVV1 is a novel member of the genus Varicosavirus of the family Rhabdoviridae. The conserved regulatory elements involved in transcription termination/polyadenylation and transcription initiation were identified in the ZaVV1 gene-junction regions with the consensus sequence 3'-UAUUAUUCUUUUUGCUCU-5' (in the negative-sense genome). The ZaVV1 genome sequence may be useful for studying the phylogenetic relationships of varicosaviruses and genome evolution of rhabdoviruses.
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