Massilia soli sp. nov., isolated from soil

Abstract

A Gram-stain-negative, catalase-and oxidase-positive and aerobic bacterium, designated strain R798T, was isolated from soil in South Korea. Cells were motile rods by means of a single polar flagellum. Growth of strain R798T was observed at 15–35 °C (optimum, 25–30 °C), pH 5.0–8.0 (optimum, 6.0) and 0–1.5% NaCl (optimum, 0.3%). Strain R798T contained ubiquinone-8 as the sole isoprenoid quinone, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C16:0 as the major fatty acids and phosphatidylglyc-erol and phosphatidylethanolamine as the major polar lipids. The DNA G+C content of strain R798T calculated from the whole genome sequence was 63.3 mol%. Phylogenetic analyses based on the 16S rRNA gene and whole genome sequences revealed that strain R798T formed a distinct phyletic lineage within the genus Massilia. Strain R798T was most closely related to Massilia eurypsychrophila B528-3T with a 98.0% 16S rRNA gene sequence similarity. Average nucleotide identity and digital DNA–DNA hybridization values between strain R798T and the type strain of M. eurypsychrophila were 79.2 and 22.7%, respectively. Based on the phenotypic, chemotaxonomic and molecular analyses, strain R798T represents a novel species of the genus Massilia, for which the name Massilia soli sp. nov. is proposed. The type strain is R798T (=KACC 22114T=JCM 34601T). © 2022 The Authors.