유방암에서 색소원제자리부합화에 의한 HER-2/neu 종양유전자 증폭과 Topoisomerase II-α 의 면역조직화학적 발현HER-2/neu Oncogene Amplification by Chromogenic in situ Hybridization and Immunohistochemical Expression of Topoisomerase II-α in the Breast Cancer
- Authors
- Lee, Tae Jin; Oh, Hyung Goon; Kwon, Gui Young; Kim, Mi Kyung; Park, Eon Sub; Yoo, Jae Hyung
- Issue Date
- Feb-2003
- Publisher
- 대한병리학회
- Keywords
- Mammary Neoplasms-In SituHybridization-HER-2/neu-DNA Topoisomerase-type II-α
- Citation
- Journal of Pathology and Translational Medicine, v.37, no.1, pp 26 - 34
- Pages
- 9
- Journal Title
- Journal of Pathology and Translational Medicine
- Volume
- 37
- Number
- 1
- Start Page
- 26
- End Page
- 34
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55863
- ISSN
- 2383-7837
2383-7845
- Abstract
- Background : Amplifications of the HER-2/neu oncogene and the Topoisomerase II-α gene are important determiners of the response to chemotherapy in the breast cancer. For detecting
HER-2/neu amplification, fluorescent in situ hybridization and immunohistochemistry are currently regarded as standard methods. Chromogenic in situ hybridization (CISH) is investigated
as a new modification of in situ hybridization. The purpose of this study is to compare the efficacy of CISH and immunohistochemistry (IHC) in detecting HER-2/neu oncogene amplification and to investigate the prognostic significance of the HER-2/neu oncogene and the Topoisomerase II-α gene in breast cancer. Methods : Using CISH and IHC the amplifications and protein expressions of the HER-2/neu oncogene were studied on paraffin sections of 43 infiltrating duct carcinomas. The expression of the Topoisomerase II-α gene was studied
immunohistochemically. Results : Of the 43 infiltrating duct carcinomas, amplifications of the HER-2/neu oncogene by CISH were observed in 8 cases (18.6%), and the HER-2/neu protein was deemed overexpressed by IHC in 9 cases (20.9%). The amplifications of the HER-2/neu oncogene showed a statistically significant correlation with tumor size, histological grade, and the Topoisomerase II-α index. The Topoisomerase II-α index showed a statistically significant correlation with tumor size, lymph node status, stage, histologic grade, and estrogen receptor status. Conclusions : CISH is a useful alternative for determining HER-2/neu amplification, especially for confirming the immunohistochemical staining results. HER-2/neu amplification
and the Topoisomerase II-α gene index may be prognostic factors of breast cancer.
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