Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
- Authors
- Lee ,Jaejin; Shin, Eunkyoung; Yeom, Ji-Hyun; Park, Jaeyoung; Kim, Sunwoo; Lee, Minho; Lee, Kangseok
- Issue Date
- Apr-2022
- Publisher
- Academic Press
- Keywords
- Pathogenicity; RNase E; Salmonella Typhimurium; STRraA; Virulence
- Citation
- Microbial Pathogenesis, v.165
- Journal Title
- Microbial Pathogenesis
- Volume
- 165
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56085
- DOI
- 10.1016/j.micpath.2022.105460
- ISSN
- 0882-4010
1096-1208
- Abstract
- RNase E-mediated RNA processing and degradation are involved in bacterial adaptation to environmental changes. The RraA regulatory protein, which is highly conserved in γ-proteobacteria, differentially modulates RNase E activity. Recent studies have revealed the association of Salmonella enterica serovar Typhimurium RNase E (STRNase E) with bacterial pathogenicity; however, the molecular mechanisms are unknown. Here, we show that the expression levels of STRraA, a protein regulator of STRNase E activity, affect S. Typhimurium pathogenicity. RNA-sequencing and RT-PCR analyses indicated positive effects of STRraA levels on the abundance of mRNA species from class II flagellar operons. Primer extension analysis further identified STRraA-regulated STRNase E cleavage in the 5′ untranslated region of fliDST mRNA. The cleavage affected the stability of this polycistronic mRNA, suggesting that STRraA protects fliDST mRNA from STRNase E cleavage, leading to enhanced flagellar assembly. Accordingly, STRraA positively regulated flagellar assembly and motility. In addition, STrraA-deleted cells showed decreased invasion ability and cytotoxicity in infection of human cervical epithelial carcinoma cells and reduced mortality in a mouse infection model compared to wild-type cells. These results support an active role of STRraA in RNase E-mediated modulation of pathogenesis in S. Typhimurium. © 2022
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