Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzaeopen access
- Authors
- Heo, Joo-Hyung; Ananin, Vladimir; Park, Jeong-Seok; Lee, Chung-Ryul; Moon, Jun-Ok; Kwon, Ohsuk; Kang, Hyun-Ah; Kim, Chul Ho; Rhee, Sang Ki
- Issue Date
- Jun-2004
- Publisher
- Korean Society for Biotechnology and Bioengineering
- Keywords
- Aspergillus oryzae; Fed-batch fermentation; Filamentous fungi; Morphology; Propeller agitation; Submerged culture
- Citation
- Biotechnology and Bioprocess Engineering, v.9, no.3, pp 184 - 190
- Pages
- 7
- Journal Title
- Biotechnology and Bioprocess Engineering
- Volume
- 9
- Number
- 3
- Start Page
- 184
- End Page
- 190
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57520
- DOI
- 10.1007/BF02942290
- ISSN
- 1226-8372
1976-3816
- Abstract
- The influences of impeller types on morphology and protein expression were investigated in a submerged culture of Aspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production for both the intracellularly heterologous protein (β-glucuronidase) and the extracellularly homologous protein (α-amylase). The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation of A. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged fed-batch fermentation of recombinant A. oryzae.
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