Non-AUG translational initiation of a short CAPC transcript generating protein isoformopen access
- Authors
- Anaganti, Suresh; Hansen, Johanna K.; Ha, Duc; Hahn, Yoonsoo; Chertov, Oleg; Pastan, Ira; Bera, Tapan K.
- Issue Date
- Mar-2009
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- LRRC26; Prostate cancer; Breast cancer; Alternate transcript; Non-AUG initiation
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.380, no.3, pp 508 - 513
- Pages
- 6
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 380
- Number
- 3
- Start Page
- 508
- End Page
- 513
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57569
- DOI
- 10.1016/j.bbrc.2009.01.089
- ISSN
- 0006-291X
1090-2104
- Abstract
- CAPC (also known as LRRC26) is a new gene with restricted expression in normal tissues, and with expression in many cancers and cancer cell lines. We have identified and characterized a short-transcript of CAPC(S-CAPC). The nucleotide sequence analysis of CAPC mRNA showed that the transcription for S-CAPC starts at position +610 on the L-CAPC transcript. Interestingly, no translation initiation codon 'AUG' is present in this transcript. To determine if a non-AUG start site is utilized, the S-CAPC sequence was cloned into an expression vector With C-terminal myc and histidine tags, and transfected into 293T cells. Western blot and MALDI-TOF MS analysis on purified S-CAPC gave two distinct peaks at approximately 7.5 kDa. N-terminal amino acid sequencing of the purified 7.5 kDa protein product indicated that translation starts at the codon for cysteine on the S-CAPC transcript generating a 7.5 kDa CAPC Protein products translated from a non-AUG initiation site. Published by Elsevier Inc
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