Identification and quantification of sialylated and core-fucosylated N-glycans in human transferrin by UPLC and LC-MS/MS
- Authors
- Jin, M.; Kim, J.; Ha, J.; Kim, A.; Lee, J.; Park, C.S.; Kang, M.; Kim, J.; Mun, C.; Kim, J.; Kim, H.H.
- Issue Date
- Jun-2022
- Publisher
- Academic Press Inc.
- Keywords
- Column; Core-fucosylated N-glycan; Fluorescent tag; LC-MS/MS; Sialylated N-glycan; UPLC
- Citation
- Analytical Biochemistry, v.647
- Journal Title
- Analytical Biochemistry
- Volume
- 647
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57784
- DOI
- 10.1016/j.ab.2022.114650
- ISSN
- 0003-2697
1096-0309
- Abstract
- Sialylated and core-fucosylated N-glycans in human transferrin (HTF) are used as glycan biomarkers due to their increased or decreased characteristics in certain diseases. However, their absolute quantities remain unclear. In this study, N-glycans of HTF were identified by UPLC and LC-MS/MS using fluorescence tags [2-aminobenzamide (AB) and procainamide (ProA)] and columns [HILIC and anion exchange chromatography-HILIC (AXH)]. The structures of 14 (including five core-fucosylated) N-glycans in total comprising two non-, six mono-, four di-, and two tri-sialylated N-glycans were identified. The quantities (%) of each N-glycan relative to the total N-glycans (100%) were obtained. HILIC and AXH were better for peak identification and separability except for desialylation, respectively. Specifically, sialylated (in ProA-HILIC and ProA-AXH by UPLC or LC-MS/MS) and core-fucosylated (in AB-HILIC and ProA-AXH by UPLC) N-glycans were efficiently identified. Seven neuraminidase-treated (including three core-fucosylated) N-glycans were efficiently identified in ProA-AXH, even their poor separation. Additionally, ProA-AXH was more efficient for the estimation of the absolute quantities of N-glycans from the results of fluorescence intensity (by UPLC) and relative quantity (by LC-MS/MS). These results first demonstrate that ProA is useful for identifying and quantifying sialylated, core-fucosylated, and neuraminidase-treated desialylated N-glycans in HTF using AXH by UPLC and LC/MS. © 2022 Elsevier Inc.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57784)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.