Histone Acetyltransferase-dependent Chromatin Remodeling and Vascular Clockopen access히스톤 아세틸화에 의한 크로마틴 재구성과 순환기의 생물학적시계
- Authors
- Curtis, Anne M.; Seo, Sang-beom; Westgate, Elizabeth J.; Rudic, Radu Daniel; Smyth, Emer M.; Chakravarti, Debabrata; FitzGerald, Garret A.; McNamara, Peter
- Issue Date
- Feb-2004
- Publisher
- American Society for Biochemistry and Molecular Biology Inc.
- Citation
- Journal of Biological Chemistry, v.279 , no.8, pp 7091 - 7097
- Pages
- 7
- Journal Title
- Journal of Biological Chemistry
- Volume
- 279
- Number
- 8
- Start Page
- 7091
- End Page
- 7097
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58159
- DOI
- 10.1074/jbc.M311973200
- ISSN
- 0021-9258
1083-351X
- Abstract
- Rhythmic gene expression is central to the circadian control of physiology in mammals. Transcriptional activation of Per and Cry genes by heterodimeric bHLH-PAS proteins is a key event in the feedback loop that drives rhythmicity; however, the mechanism is not clearly understood. Here we show the transcriptional coactivators and histone acetyltransferases, p300/CBP, PCAF, and ACTR associate with the bHLH-PAS proteins, CLOCK and NPAS2, to regulate positively clock gene expression. Furthermore, Cry2 mediated repression of NPAS2:BMAL1 is overcome by overexpression of p300 in transactivation assays. Accordingly, p300 exhibits a circadian time-dependent association with NPAS2 in the vasculature, which precedes peak expression of target genes. In addition, a rhythm in core histone H3 acetylation on the mPer1 promoter in vivo correlates with the cyclical expression of their mRNAs. Temporal coactivator recruitment and HAT-dependent chromatin remodeling on the promoter of clock controlled genes in the vasculature permits the mammalian clock to orchestrate circadian gene expression.
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