Square microchannel enables to focus and orient ellipsoidal Euglena gracilis cells by two-dimensional acoustic standing wave
- Authors
- Park, Sungryul; Lee, Sangwook; Kim, Hyun Soo; Choi, Hong Jin; Jeong, Ok Chan; Lin, Ruixian; Cho, Younghak; Lee, Min Ho
- Issue Date
- Sep-2022
- Publisher
- SPRINGER WIEN
- Keywords
- Acoustofluidics; Microalgae; Acoustic focusing; Microfluidics; Flow cytometry
- Citation
- MICROCHIMICA ACTA, v.189, no.9
- Journal Title
- MICROCHIMICA ACTA
- Volume
- 189
- Number
- 9
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58882
- DOI
- 10.1007/s00604-022-05439-7
- ISSN
- 0026-3672
1436-5073
- Abstract
- Flow cytometry has become an indispensable tool for counting, analyzing, and sorting large cell populations in biological research and medical practice. Unfortunately, it has limitations in the analysis of non-spherically shaped cells due to the variation of their alignment with respect to the flow direction and, hence, the optical interrogation axis, resulting in unreliable cell analysis. Here, we present a simple on-chip acoustofluidic method to fix the orientation of ellipsoidal cells and focus them into a single, aligned stream. Specifically, by generating acoustic standing waves inside a 100 . 100 mu m square-shaped microchannel, we successfully aligned and focused up to 97.7% of a population of Euglena gracilis (an ellipsoidal shaped microalgal species) cells in the center of the microchannel with high precision at a volume rate of 25 to 200 mu L min(-1). Uniform positioning of ellipsoidal cells is essential for making flow cytometry applicable to the investigation of a greater variety of cell populations and is expected to be beneficial for ecological studies and aquaculture.
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