Effect of IgG Fc-fusion and KDEL-ER retention signal on prostate-specific antigen expression in plant and its immune in mice
- Authors
- Shin, Chunha; Kim, Kibum; Kang, Yang Joo; Kim, Deuk-Su; Seo, Young-Jin; Park, Se Ra; Kim, Mi Kyung; Lee, Young Koung; Kim, Do-Sun; Ko, Kisung
- Issue Date
- Dec-2022
- Publisher
- Springer
- Keywords
- Fc fusion protein; Plant; Prostate-specific antigen; Therapeutic protein; Vaccine candidate
- Citation
- Plant Biotechnology Reports, v.16, no.6, pp 729 - 740
- Pages
- 12
- Journal Title
- Plant Biotechnology Reports
- Volume
- 16
- Number
- 6
- Start Page
- 729
- End Page
- 740
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/60543
- DOI
- 10.1007/s11816-022-00810-9
- ISSN
- 1863-5466
1863-5474
- Abstract
- Prostate-specific antigen (PSA) is a protein highly expressed in cancer cells of the prostate gland. In this study, the PSA recombinant protein was fused to the human immunoglobulin G crystallizable fragment (Fc) with the endoplasmic reticulum (ER) Lys-Asp-Glu-Leu (KDEL) retention signal tag for the plant expression system. Agrobacterium-mediated plant transformation was applied to generate transgenic tobacco plants expressing PSA tagged to KDEL (PSAK), PSA-Fc, and PSA-Fc fused to KDEL (PSA-FcK). Western blot analysis showed that the PSA-FcK protein was the most highly expressed among the three different proteins in the transgenic plant leaf. The PSAK, PSA-Fc, and PSA- FcK proteins were successfully purified from plant leaves. Reduced and non-reduced sodium dodecyl sulfate–polyacrylamide gel electrophoresis revealed that the PSA proteins fused to Fc and FcK were dimerized, whereas the PSA itself was not dimerized. N-glycan analysis demonstrated that the N-glycan profile of the PSA-Fc was mainly the structure with α(1,3)-fucose (Fuc) or β(1,2)-xylose (Xyl) glycan residues, whereas the PSA-Fc tagged with the KDEL ER retention signal had mainly an oligomannose-type structure. ELISA and SPR showed that PSA-FcK had a higher binding activity to Fc gamma receptor I than PSA-Fc and human Fc. In addition, PSA-FcK induced anti-PSA, and thus has the potential to be utilized as a prostate cancer vaccine. © 2022, Korean Society for Plant Biotechnology.
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Collections - College of Medicine > College of Medicine > 1. Journal Articles
- College of Natural Sciences > Department of Life Science > 1. Journal Articles
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