High performance inkjet printed embedded electrochemical sensors for monitoring hypoxia in a gut bilayer microfluidic chip
- Authors
- Khalid, Muhammad Asad Ullah; Kim, Kyung Hwan; Salih, Abdul Rahim Chethikkattuveli; Hyun, Kinam; Park, Sung Hyuk; Kang, Bohye; Soomro, Afaque Manzoor; Ali, Muhsin; Jun, Yesl; Huh, Dongeun; Cho, Heeyeong; Choi, Kyung Hyun
- Issue Date
- May-2022
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- LAB ON A CHIP, v.22, no.9, pp 1764 - 1778
- Pages
- 15
- Journal Title
- LAB ON A CHIP
- Volume
- 22
- Number
- 9
- Start Page
- 1764
- End Page
- 1778
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/61637
- DOI
- 10.1039/d1lc01079d
- ISSN
- 1473-0197
1473-0189
- Abstract
- Sensing devices have shown tremendous potential for monitoring state-of-the-art organ chip devices. However, challenges like miniaturization while maintaining higher performance, longer operating times for continuous monitoring, and fabrication complexities limit their use. Herein simple, low-cost, and solution-processible inkjet dispenser printing of embedded electrochemical sensors for dissolved oxygen (DO) and reactive oxygen species (ROS) is proposed for monitoring developmental (initially normoxia) and induced hypoxia in a custom-developed gut bilayer microfluidic chip platform for 6 days. The DO sensors showed a high sensitivity of 31.1 nA L mg(-1) with a limit of detection (LOD) of 0.67 mg L-1 within the 0-9 mg L-1 range, whereas the ROS sensor had a higher sensitivity of 1.44 nA mu m(-1) with a limit of detection of 1.7 mu m within the 0-300 mu m range. The dynamics of the barrier tight junctions are quantified with the help of an in-house developed trans-epithelial-endothelial electrical impedance (TEEI) sensor. Immunofluorescence staining was used to evaluate the expressions of HIF-1 alpha and tight junction protein (TJP) ZO-1. This platform can also be used to enhance bioavailability assays, drug transport studies under an oxygen-controlled environment, and even other barrier organ models, as well as for various applications like toxicity testing, disease modeling and drug screening.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Engineering > School of Mechanical Engineering > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/61637)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.