Inhibitory effect of conditioned medium of silk fibroin-treated osteoblasts in osteoclast differentiation
- Authors
- Yeo, J.-H.; Park, K.-H.; Ju, W.-C.; Lee, J.; Lee, K.-G.; Woo, S.-O.; Han, S.-M.; Kweon, H.; Kim, S.-S.; Cho, Y.
- Issue Date
- 2008
- Publisher
- Korean Society of Food Science and Nutrition
- Keywords
- Conditioned medium; Interleukin-1β; Osteoclastic differentiation; Osteoprotegerin; Silk-fibroin
- Citation
- Journal of the Korean Society of Food Science and Nutrition, v.37, no.8, pp 992 - 997
- Pages
- 6
- Journal Title
- Journal of the Korean Society of Food Science and Nutrition
- Volume
- 37
- Number
- 8
- Start Page
- 992
- End Page
- 997
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65346
- DOI
- 10.3746/jkfn.2008.37.8.992
- ISSN
- 1226-3311
2288-5978
- Abstract
- In this study, we investigated the indirect effect of silk-fibroin on osteoclastic differentiation of RAW264.7 cells. The conditioned medium were collected from MC3T3-E1 osbeoblasts treated with 0.001 mg/mL~0.1 mg/mL silk fibroin for 6 days, mixed in 1:1 ratio with osteoclast medium, and then added into RAW264.7 cells with receptor activator of nuclear factor kappa B ligand (RANKL), a differentiation inducer for 3 days. Of osteoclastic cytokines in the conditioned medium, the protein expression of osteoprotegerin (OPG) with silk-fibroin was not significantly different. However, the protein expression of interleukin (IL)-1β was specifically lower in a dose dependent manner. In RAW264.7 cells, the conditioned medium with silk-fibroin inhibited RANKL induced osteoclastic differentiation as total number of multinucleated tartrate-resistant alkaline phosphatase (TRAP)-positive osteoclasts in a dose dependent manner. Taken together, we demonstrated that the conditioned medium of silk-fibroin treated osteoblasts inhibits RANKL induced differentiation of osteoclasts with inhibiting selective expression of IL-1β.
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