Expression of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and Fc fragment of antibody using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leavesopen access
- Authors
- Kim, Kyung Il; Chung, Ha Young; Yoo, Ki Hyun; Park, Jong-Hwa; Lee, Hyun Ho; Soek, Yeon Ju; Ko, Ki Sung; Kang, Hyung Sik; Lee, Kyung Jin; Oh, Doo-Byoung; Joung, Young Hee; Chung, In Sik
- Issue Date
- Jul-2012
- Publisher
- SPRINGER
- Keywords
- Colorectal cancer; GA733-2; Nicotiana benthamiana; BCTV (Beet curly top virus); Vaccination
- Citation
- PLANT BIOTECHNOLOGY REPORTS, v.6, no.3, pp 233 - 242
- Pages
- 10
- Journal Title
- PLANT BIOTECHNOLOGY REPORTS
- Volume
- 6
- Number
- 3
- Start Page
- 233
- End Page
- 242
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66688
- DOI
- 10.1007/s11816-012-0218-3
- ISSN
- 1863-5466
1863-5474
- Abstract
- We describe expression and characterization of recombinant human colorectal cancer antigen GA733-2 fused to Fc fragment of antibody (GA733-2-Fc) using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leaves. Recombinant GA733-2-Fc/KDEL with a molecular mass of similar to 68 kDa was transiently expressed. The level of expression of GA733-2-Fc with ER retention signal KDEL (GA733-2-Fc/KDEL) in the expression vector system was 0.96% of total soluble proteins. Recombinant GA733-2-Fc/KDEL was purified using an affinity chromatography. Mice immunized with recombinant GA733-2-Fc/KDEL mounted a strong GA733-2-Fc/KDEL-specific serum antibody response. Vaccination of plant-derived recombinant GA733-2-Fc/KDEL regressed tumor volumes in BALB/c mice. The population of activated-T and NK-T cells increased notably in lymph node, spleen, and tumor-infiltrating lymphocytes derived from the tumor-regressed mice. Taken together, recombinant GA733-2-Fc/KDEL expressed in plants can be used as an effective experimental immunogen for research in cancer vaccine development.
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