Higher expression of TRPM7 channels in murine mature B lymphocytes than immature cells
- Authors
- Kim, Jin Kyoung; Ko, Jae Hong; Nam, Joo Hyun; Woo, Ji Eun; Min, Kyeong Min; Earm, Yung E; Kim, Sung Joon
- Issue Date
- Apr-2005
- Publisher
- 대한약리학회
- Keywords
- B lymphocyte; Bal-17; Cell death; Nonselective cation channel; TRPM7; WEHI-231
- Citation
- The Korean Journal of Physiology & Pharmacology, v.9, no.2, pp 69 - 75
- Pages
- 7
- Journal Title
- The Korean Journal of Physiology & Pharmacology
- Volume
- 9
- Number
- 2
- Start Page
- 69
- End Page
- 75
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66794
- ISSN
- 1226-4512
2093-3827
- Abstract
- TRPM7, a cation channel protein permeable to various metal ions such as Mg2+, is ubiquitously expressed in variety of cells including lymphocytes. The activity of TRPM7 is tightly regulated by intracellular Mg2+, thus named Mg2+-inhibited cation (MIC) current, and its expression is known to be critical for the viability and proliferation of B lymphocytes. In this study, the level of MIC current was compared between immature (WEHI-231) and mature (Bal-17) B lymphocytes. In both cell types, an intracellular dialysis with Mg2+-free solution (140 mM CsCl) induced an outwardly-rectifying MIC current. The peak amplitude of MIC current and the permeability to divalent cation (Mn2+) were several fold higher in Bal-17 than WEHI-231. Also, the level of mRNAs for TRPM7, a molecular correspondence of the MIC channel, was significantly higher in Bal-17 cells. The amplitude of MIC was further increased, and the relation between current and voltage became linear under divalent cation-free conditions, demonstrating typical properties of the TRPM7. The stimulation of B cell receptors (BCR) by ligation with antibodies did not change the amplitude of MIC current. Also, increase of extracellular [Mg2+]c to enhance the Mg2+ influx did not affect the BCR ligation-induced death of WEHI-231 cells. Although the level of TRPM7 was not directly related with the cell death of immature B cells, the remarkable difference of TRPM7 might indicate a fundamental change in the permeability to divalent cations during the development of B cells.
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