Sphingosine-1-phosphate promotes mouse melanocyte survival via ERK and Akt activation
- Authors
- Kim, Dong-Seok; Hwang, Eui-Soo; Lee, Jai-Eun; Kim, Sook-Young; Park, Kyoung-Chan
- Issue Date
- Oct-2003
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- sphingosine-1-phosphate; UV; cytoprotection; proliferation; ERK; Akt
- Citation
- CELLULAR SIGNALLING, v.15, no.10, pp 919 - 926
- Pages
- 8
- Journal Title
- CELLULAR SIGNALLING
- Volume
- 15
- Number
- 10
- Start Page
- 919
- End Page
- 926
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66862
- DOI
- 10.1016/S0898-6568(03)00055-x
- ISSN
- 0898-6568
1873-3913
- Abstract
- In this study, we investigated the signalling pathways induced by ultraviolet B (UVB) and the effects of sphingosine-1-phosphate on UVB-induced apoptosis of mouse melanocytes, Mel-Ab, and observed the cytoprotective effects of sphingosine-1-phosphate on UVB-induced apoptosis. Since sphingosine-1-phosphate is a well-known mitogenic agent, we thought it possible that the mitogenic effect of sphingosine-1-phosphate might contribute to cell survival. However, we found that sphingosine-1-phosphate significantly inhibits DNA synthesis. We next examined the regulation of the three major subfamilies of mitogen-activated protein (MAP) kinases and of the Akt pathway by sphingosine-1-phosphate against UVB-induced apoptosis. UVB irradiation resulted in the remarkable and sustained activation of c-Jun N-terminal kinase (INK), while p38 MAP kinase was only transiently activated. The basal level of extracellular signal-regulated protein kinase (ERK) phosphorylation decreased 30 min after UVB irradiation, whereas the basal level of Akt phosphorylation was unaffected by UVB. We also found that sphingosine-1-phosphate potently stimulates the phosphorylation of both ERK and Akt, which are involved in the cell survival-signalling cascade. Furthermore, the specific inhibition of the ERK and Akt pathways by PD98059 and LY294002, respectively, restored the cytoprotective effect induced by sphingosine-1-phosphate. On the other hand, the p38 inhibitor SB203580 additively enhanced the cytoprotective effect on sphingosine-1-phosphate. Based on these results, we conclude that the activation of p38 MAP kinase plays an important role in UVB-induced apoptosis, and that sphingosine-1-phosphate probably exert its cytoprotective effect in Mel-Ab cells through ERK and Akt activation. (C) 2003 Elsevier Science Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.