자궁경부 상피내 종양과 침윤성 편평상피암종에서 claudin-1, claudin-4, zonula occludens-1의 발현Expression of claudin-1, claudin-4 and zonula occludens-1 in cervical intraepithelial neoplasia and invasive squamous cell carcinoma
- Authors
- 이선경; 노현경; 박태양; 강규현; 조태일; 이태진
- Issue Date
- Oct-2007
- Publisher
- 대한산부인과학회
- Keywords
- Claudin-1; Claudin-4; ZO-1; CIN; Cervical Cancer; Claudin-1; Claudin-4; ZO-1; 자궁경부 상피내 종양; 자궁경부암
- Citation
- Obstetrics & Gynecology Science, v.50, no.10, pp 1378 - 1385
- Pages
- 8
- Journal Title
- Obstetrics & Gynecology Science
- Volume
- 50
- Number
- 10
- Start Page
- 1378
- End Page
- 1385
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67304
- ISSN
- 2287-8572
2287-8580
- Abstract
- =목적: 종양의 발생과 진행 과정에서 폐쇄막를 구성하는 단백질인 claudins와 ZO가 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 자궁경부의 상피내 종양과 ISCC에서 claudin-1, claudin-4, ZO-1의 발현 양상의 차이점을 알아보고자 하였다.연구 방법: 자궁경부의 생검이나 원뿔절제술을 통하여 LSIL로 진단된 22예, HSIL로 진단된 36예와 자궁적출술을 통하여 ISCC로 진단된 20예의 파라핀 포매 조직에서 면역조직화학 염색법을 이용하여 claudin-1, claudin-4, ZO-1의 발현을 분석하였다.결과: claudin-1은 LSIL의 40.9%, HSIL의 94.4%, ISCC의 20.0%에서 양성 발현이 관찰되어 HSIL에서 유의하게 높았으며 (p=0.0001), claudin-4는 LSIL의 31.8%, HSIL의 41.7%, ISCC의 25.0%에서 양성 발현이 되어 HSIL에서 가장 높았으나, 통계학적 유의성은 없었다. ZO-1은 LSIL의 13.6%, HSIL의 41.7%, ISCC의 10.0%에서 양성 발현이 관찰되어 HSIL에서 유의하게 높았다 (p=0.011).
Objective: Cell to cell and cell to extracellular matrix interaction are crucial in tumor development and progression. Tight junction proteins
such as claudins and zonula occludens-1 (ZO-1) play an important role in these processes. This study was performed to investigate the
difference of expressions of claudin-1, claudin-4 and ZO-1 in low grade squamous intraepithelial lesion (LSIL), high grade squamous
intraepithelial lesion (HSIL), and invasive squamous cell carcinoma (ISCC) of the uterine cervix.·
Methods: The expressions of claudin-1, claudin-4 and ZO-1 were evaluated using immunohistochemical staining in 78 cervical tissue
specimens (LSIL 22 case, HSIL 36 case, and ISCC 20 case).
Results: Claudin-1 expression was positive in 40.9% of LSIL, in 94.0% of HSIL and in 20.0% of ISCC. The expression of claudin-1 was
significantly high in HSIL (p=0.0001). Claudin-4 expression was positive in 31.8% of LSIL, in 41.7% of HSIL and in 25.0% of ISCC.
The expression of claudin-4 was high in HSIL, but it was not statistically different. ZO-1 expression was positive in 13.6% of LSIL,
in 41.7% of HSIL, and in 25.5% of ISCC. The expression of ZO-1 was significantly high in HSIL (p=0.011).
Conclusion: These results indicate increased expressions of claudin-1 and ZO-1 in the HSIL that includes cervical intraepithelial neoplasia
(CIN) 2 and 3, which decrease during progression to cervical cancer.
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