Comparative responses of sulforaphene contents between radish (Raphanus sativus L.) and Baemuchae (xBrassicoraphanus) during seed development

Abstract

Sulforaphene is an isothiocyanate which is produced by the hydrolysis from glucoraphenin, one of glucosinolates mostly found in radish plant. This study evaluates the genetic diversity of sulforaphene contents from the seedlings of 29 radish genotypes in an attempt to find a variety with a high extraction yield and which can be used as a breeding source of intergeneric hybrid vegetable Baemuchae. The sulforaphene content was highest in the seedlings of ‘Cheongwoonplus’ (1,426.8 mg kg− 1 FW) and ‘Taebaek’ (1,363.3 mg kg− 1 FW) cultivars and lowest in the ‘Matchuimbom’ (328.7 mg kg− 1 FW) cultivar. The sulforaphene contents of four radish cultivars and three Baemuchae varieties sharply decreased during seedling growth; however, they were much higher in the seedlings of radish cultivars than in Baemuchae varieties. The sulforaphene contents in unpollinated pistil tissues were not affected by species, whereas they responded differently to the cultivar or variety. After pollination, the sulforaphene content gradually increased in radish cultivars during seed development, but relatively much less in Baemuchae varieties. These results indicated that the sulforaphene content varied widely in the seedlings of radish cultivars. Sulforaphene gradually accumulated after pollination during seed development in radish cultivars, but not much in Baemuchae varieties. However, not much difference was observed in the sulforaphene contents of unpollinated pistil tissues of radish and baemoochae. © 2023, The Author(s), under exclusive licence to Korean Society for Horticultural Science.