Expression of tumour necrosis factor-α is associated with apoptosis in lungs of pigs experimentally infected with porcine reproductive and respiratory syndrome virus
- Authors
- Choi, C; Chae, C
- Issue Date
- Feb-2002
- Publisher
- W B SAUNDERS CO LTD
- Citation
- RESEARCH IN VETERINARY SCIENCE, v.72, no.1, pp 45 - 49
- Pages
- 5
- Journal Title
- RESEARCH IN VETERINARY SCIENCE
- Volume
- 72
- Number
- 1
- Start Page
- 45
- End Page
- 49
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68804
- DOI
- 10.1053/rvsc.2001.0519
- ISSN
- 0034-5288
1532-2661
- Abstract
- Expression of tumour necrosis factor (TNF)-alpha and its association with apoptosis was examined in lungs from pig experimentally inoculated with porcine reproductive and respiratory syndrome virus (PRRSV). The TNF-alpha nucleic acid was detected in PRRSV-infected alveolar macrophages by reverse transcription-polymerase chain reaction (RT-PCR). The TNF-a antigen was detected in alveolar macrophages by immunohistochemisty. DNA fragmentation in apoptotic cells was detected by in situ terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL.). A double-labelling procedure using immunohistochemisty for the detection of PPRSV and the in situ TUNEL assay for the detection of apoptosis demonstrated that a majority of labelled cells were infected with PRRSV or apoptotic, but not both. Apoptotic cells were more abundant than PRRSV-infected cells in all lungs examined. A double-labelling procedure using immunohistochemisty for the detection of TNF-alpha and the in Situ TUNEL assay for the detection of apoptosis demonstrated that a majority of labelled cells were apoptotic or for TNF-alpha but not both. These data suggested that TNF-alpha released from macrophages after infection by PRRSV may induce apoptosis in uninfected bystander cells. (C) 2002 Harcourt Publishers Ltd.
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