The stabilization of lactic acid bacteria, Bacillus polyfermenticus KJS-2

Abstract

Bacillus polyfermenticus KJS-2 (Bp2) was isolated from Bispan®, a commercially available probiotics consisting of more than 4 strains. The objective of this study was to investigate the effect of three-layer coating on the stabilty of Bp2. Bp2 was microencapsulated with sodium alginate using an air atomizer. The Bp2 loaded pellets were also coated with HFP-chitosan and HPMCP for oral delivery system. When compared to the uncoated Bp2, the survival of the three-layer coated Bp2 increased to approximately 63% (p<0.01) in a 30% ethanol solution, 54% (p<0.05) in an artificial gastric juice (pH 2), and 53% (p<0.05) in the bile acid (pH 5). When coated beads were stored at 100°C and 130°C, Bp2 in coated beads was very stable (p<0.01) compared to uncoated Bp2.