Determination of Mycoplasma hyopneumoniae-Specific IgG, IgG1, and IgG2a Titers in BALB/c Mice Induced by Mineral Oil-Based Oil-in-Water Emulsion Adjuvants Prepared Using a Self-Emulsifying Drug Delivery System
- Authors
- Bastola, Rakesh; Seo, Jo-Eun; Noh, Gyubin; Keum, Taekwang; Kim, Ju Hun; Shin, Jong Il; Lee, Sooyeun; Lee, Sangkil
- Issue Date
- Jan-2019
- Publisher
- SPRINGER
- Keywords
- self-emulsifying drug delivery system; mineral oil-based emulsion adjuvant; Mycoplasma hyopneumoniae-specific IgG; IgG1; and IgG2a titers
- Citation
- AAPS PHARMSCITECH, v.20, no.1
- Journal Title
- AAPS PHARMSCITECH
- Volume
- 20
- Number
- 1
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69428
- DOI
- 10.1208/s12249-018-1245-3
- ISSN
- 1530-9932
1522-1059
- Abstract
- We prepared mineral oil-based emulsion adjuvants by employing simple self-emulsifying drug delivery system (SEDDS). Mineral oil emulsions (3%, 5%, and 7%) were prepared using deionized water and C-971P NF and C-940 grade carbomer solutions with concentrations 0.01% (w/v) and 0.02% (w/v). In total, 15 emulsions were prepared and mixed with a solution containing inactivated Mycoplasma hyopneumoniae (J101 strain) antigen and porcine circovirus type 2 antigen to prepare vaccines. Droplet sizes in the submicron range and zeta potential values between -40 and 0mV were maintained by most emulsion adjuvants for a period of 6months. Emulsion adjuvants were regarded safe, and their M. hyopneumoniae-specific IgG, IgG1, and IgG2a titers were either better or comparable to those of aluminum gel.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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