A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cellsopen access
- Authors
- Kim, Hee-Yeon; Lee, Younghay; Yoon, Hee-Soo; Kim, Yu-Hee; Cho, Kyong-A; Woo, So-Youn; Kim, Han Sun; Park, Bo-Young; Jung, Sung-Chul; Jo, Inho; Park, Woo-Jae; Park, Joo-Won; Ryu, Kyung-Ha
- Issue Date
- Apr-2021
- Publisher
- KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
- Keywords
- Estrogen; Tonsil; Mesenchymal stem cell; Secretion; Cytochrome P450 family 19 subfamily A member 1
- Citation
- TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.18, no.2, pp 253 - 264
- Pages
- 12
- Journal Title
- TISSUE ENGINEERING AND REGENERATIVE MEDICINE
- Volume
- 18
- Number
- 2
- Start Page
- 253
- End Page
- 264
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69617
- DOI
- 10.1007/s13770-020-00307-y
- ISSN
- 1738-2696
2212-5469
- Abstract
- BACKGROUND: The advantages of tonsil-derived mesenchymal stem cells (TMSCs) over other mesenchymal stem cells (MSCs) include higher proliferation rates, various differentiation potentials, efficient immune-modulating capacity, and ease of obtainment. Specifically, TMSCs have been shown to differentiate into the endodermal lineage. Estrogen deficiency is a major cause of postmenopausal osteoporosis and is associated with higher incidences of ischemic heart disease and cerebrovascular attacks during the postmenopausal period. Therefore, stem cell-derived, estrogen-secreting cells might be used for estrogen deficiency. METHODS: Here, we developed a novel method that utilizes retinoic acid, insulin-like growth factor-1, basic fibroblast growth factor, and dexamethasone to evaluate the differentiating potential of TMSCs into estrogen-secreting cells. The efficacy of the novel differentiating method for generation of estrogen-secreting cells was also evaluated with bone marrow- and adipose tissue-derived MSCs. RESULTS: Incubating TMSCs in differentiating media induced the gene expression of cytochrome P450 19A1 (CYP19A1), which plays a key role in estrogen biosynthesis, and increased 17 beta-estradiol secretion upon testosterone addition. Furthermore, CYP11A1, CYP17A1, and 3 beta-hydroxysteroid dehydrogenase type-1 gene expression levels were significantly increased in TMSCs. In bone marrow-derived and adipose tissue-derived MSCs, this differentiation method also induced the gene expression of CYP19A1, but not CYP17A1, suggesting TMSCs are a superior source for estrogen secretion. CONCLUSION: These results imply that TMSCs can differentiate into functional estrogen-secreting cells, thus providing a novel, alternative cell therapy for estrogen deficiency.
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