Functional Equivalency in Human Somatic Cell Nuclear Transfer-Derived Endothelial Cells
- Authors
- Park, Soon-Jung; Lee, Ji-Heon; Lee, Seul-Gi; Lee, Jeoung Eun; Seo, Joseph; Choi, Jong Jin; Jung, Taek-Hee; Chung, Eun-Bin; Kim, Ha Na; Ju, Jongil; Song, Yun-Ho; Chung, Hyung-Min; Lee, Dong Ryul; Moon, Sung-Hwan
- Issue Date
- May-2019
- Publisher
- OXFORD UNIV PRESS
- Keywords
- Human somatic cell nuclear transfer; Endothelial cell; Ischemia; Cell transplantation; Cell therapy
- Citation
- STEM CELLS, v.37, no.5, pp 623 - 630
- Pages
- 8
- Journal Title
- STEM CELLS
- Volume
- 37
- Number
- 5
- Start Page
- 623
- End Page
- 630
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69807
- DOI
- 10.1002/stem.2986
- ISSN
- 1066-5099
1549-4918
- Abstract
- The derivation of human embryonic stem cells (hESCs) by somatic cell nuclear transfer (SCNT) has prompted a re-emerging interest in using such cells for therapeutic cloning. Despite recent advancements in derivation protocols, the functional potential of CHA-NT4 derived cells is yet to be elucidated. For this reason, this study sought to differentiate CHA-NT4 cells toward an endothelial lineage in order to evaluate in vitro and in vivo functionality. To initial differentiation, embryoid body formation of CHA-NT4 was mediated by concave microwell system which was optimized for hESC-endothelial cell (EC) differentiation. The isolated CD31+ cells exhibited hallmark endothelial characteristics in terms of morphology, tubule formation, and ac-LDL uptake. Furthermore, CHA-NT4-derived EC (human nuclear transfer [hNT]-ESC-EC) transplantation in hind limb ischemic mice rescued the hind limb and restored blood perfusion. These findings suggest that hNT-ESC-EC are functionally equivalent to hESC-ECs, warranting further study of CHA-NT4 derivatives in comparison to other well established pluripotent stem cell lines. This revival of human SCNT-ESC research may lead to interesting insights into cellular behavior in relation to donor profile, mitochondrial DNA, and oocyte quality.
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