Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast
- Authors
- Kim, Kyoung-Dong; Kim, Hyo-Jin; Lee, Kyung-Chang; Roe, Jung-Hye
- Issue Date
- May-2011
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Monothiol glutaredoxin; Fep1; Iron homeostasis; Fission yeast; CGFS motif
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.408, no.4, pp 609 - 614
- Pages
- 6
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 408
- Number
- 4
- Start Page
- 609
- End Page
- 614
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69969
- DOI
- 10.1016/j.bbrc.2011.04.069
- ISSN
- 0006-291X
1090-2104
- Abstract
- The fission yeast Schizosaccharomyces pombe contains two CGFS-type monothiol glutaredoxins. Grx4 and Grx5, which are localized primarily in the nucleus and mitochondria, respectively. We observed involvement of Grx4 in regulating iron-responsive gene expression, which is modulated by a repressor Fep1. Lack of Grx4 caused defects not only in growth but also in the expression of both iron-uptake and iron-utilizing genes regardless of iron availability. In order to unravel how Grx4 is involved in Fep1-mediated regulation, interaction between them was investigated. Co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) revealed that Grx4 physically interacts with Fep1 in vivo. BiFC revealed localized nuclear dots produced by interaction of Grx4 with Fep1. Mutation of cysteine-172 in the CGFS motif to serine (C172S) produced effects similarly observed under Grx4 depletion, such as the loss of iron-dependent gene regulation and the absence of nuclear dots in BiFC analysis. These results suggest that the ability of Grx4 to bind iron, most likely Fe-S cofactor, could be critical in interacting with and modulating the activity of Fep1. (C) 2011 Elsevier Inc. All rights reserved.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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