Primary bone-derived cells induce osteogenic differentiation without exogenous factors in human embryonic stem cells
- Authors
- Ahn, Seong Eun; Kim, Sinae; Park, Kyu Hyung; Moon, Sung Hwan; Lee, Hae Jin; Kim, Gi Jin; Lee, Young Jae; Park, Keun Hong; Cha, Kwang Yul; Chung, Hyung Min
- Issue Date
- Feb-2006
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- human embryonic stem cells; primary bone-derived cells; coculture; osteogenic differentiation
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.340, no.2, pp 403 - 408
- Pages
- 6
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 340
- Number
- 2
- Start Page
- 403
- End Page
- 408
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69986
- DOI
- 10.1016/j.bbrc.2005.12.020
- ISSN
- 0006-291X
1090-2104
- Abstract
- We developed a new and efficient method for osteoblastic differentiation of human embryonic stem cells (hESCs) using primary bone-derived cells (PBDs). Three days after embryoid body (hEB) formation, cells were allowed to adhere to culture surface where PBDs were pre-plated and mitomycin C-treated in DMEM/F12 medium supplemented with 5% knockout serum replacement, As early as 14 days, mineralization and formation of nodule-like structures in cocultured hEBs were prominent by von Kossa and Alizarin S staining, and expressions of osteoblast-specific markers including bone sialoprotein, alkaline phosphates, osteocalcin, collagen 1, and core binding factor alpha 1 by RT-PCR. In addition, FACS analysis revealed that over 19% of the differentiated cells expressed osteocalcin. These results suggest that PBDs not only have osteogenic effects releasing osteogenic factors as bone morphogenic protein (BMP) 2 and BMP 4 but also have exerted other effects, whether chemical or physical, for the differentiation of hESCs. (c) 2005 Elsevier Inc. All rights reserved.
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