Biologically synthesis of gold nanoparticles using Cirsium japonicum var. maackii extract and the study of anti-cancer properties on AGS gastric cancer cellsopen access
- Authors
- Mi, X.-J.; Park, H.-R.; Dhandapani, S.; Lee, Sanghyun; Kim, Y.-J.
- Issue Date
- 2022
- Publisher
- Ivyspring International Publisher
- Keywords
- Cirsium japonicum; Ferroptosis; Gastric cancer; Gold nanoparticles
- Citation
- International Journal of Biological Sciences, v.18, no.15, pp 5809 - 5826
- Pages
- 18
- Journal Title
- International Journal of Biological Sciences
- Volume
- 18
- Number
- 15
- Start Page
- 5809
- End Page
- 5826
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/70146
- DOI
- 10.7150/ijbs.77734
- ISSN
- 1449-2288
- Abstract
- Plant extract-mediated synthesis of metal nanoparticles (NPs) is an eco-friendly and cost-effective biosynthesis method that is more suitable for biological applications than chemical ones. We prepared novel gold NPs (AuNPs), Cirsium japonicum mediated-AuNPs (CJ-AuNPs), using a biosynthetic process involving Cirsium japonicum (Herba Cirsii, CJ) ethanol extract. The physicochemical properties of CJ-AuNPs were characterized using spectrometric and microscopic analyses. The in vitro stability of CJ-AuNPs was studied for 3 months. Moreover, the selective human gastric adenocarcinoma (AGS) cell killing ability of CJ-AuNPs was verified in cancer and normal cells. An in vitro study revealed that CJ-AuNPs trigger oxidative stress and iron-dependent ferroptosis in AGS cells. Mechanistically, CJ-AuNPs induced mitochondrial reactive oxygen species (ROS), Fe2+, and lipid peroxidation accumulation, and mitochondrial damage by destroying the glutathione peroxidase-4 (GPX4)-dependent antioxidant capacity. Furthermore, in a xenograft mouse model implanted with AGS cells, treatment with 2.5, 5, and 10 mg/kg CJ-AuNPs for 16 days reduced tumor xenograft growth in a dose dependent manner in vivo without systemic toxicity. These results demonstrate that CJ-AuNPs exert anticancer effects in vitro and in vivo by inducing ferroptosis-mediated cancer cell death. This study, based on green-synthesized nanodrug-induced ferroptosis, provides new insight into potential developments in cancer therapies. © The author(s).
- Files in This Item
-
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/70146)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.