Identification of galacturonic acid-rich polysaccharide with intestinal immune system modulating activity via Peyer's patch from postbiotics of Phellinus linteus mycelial submerged culture
- Authors
- Suh, M.G.; Shin, H.Y.; Jeong, E.-J.; Kim, G.; Jeong, S.B.; Ha, E.J.; Choi, S.-Y.; Moon, S.-K.; Shin, K.-S.; Yu, K.-W.; Suh, H.-J.; Kim, H.
- Issue Date
- Apr-2023
- Publisher
- Elsevier B.V.
- Keywords
- Acidic polysaccharide; Activity-guided fractionation; Immune activity; Medicinal mushroom; Methylation analysis
- Citation
- International Journal of Biological Macromolecules, v.234
- Journal Title
- International Journal of Biological Macromolecules
- Volume
- 234
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/70147
- DOI
- 10.1016/j.ijbiomac.2023.123685
- ISSN
- 0141-8130
1879-0003
- Abstract
- Interests in the development and exploration of industrial applications of medicinal mushrooms as postbiotics have lately increased. We recently reported the potential use of Phellinus linteus mycelial-containing whole culture extract (PLME) prepared by submerged cultivation as a postbiotic that promotes immune system activation. Here, we aimed to isolate and structurally elucidate the active ingredients in PLME by activity-guided fractionation. The intestinal immunostimulatory activity was evaluated by bone marrow (BM) cell proliferation activity and related cytokine production in C3H-HeN mouse-derived Peyer's patch (PP) cells treated with polysaccharide fractions. The initially crude polysaccharide (PLME-CP) of PLME prepared using ethanol precipitation was further fractionated into four fractions (PLME-CP-0 to -III) by anion-exchange column chromatography. BM cell proliferation and cytokine production of PLME-CP-III were significantly improved compared to those of PLME-CP. PLME-CP-III was then fractionated into PLME-CP-III-1 and PLME-CP-III-2 by gel filtration chromatography. Based on the molecular weight distribution, monosaccharide, and glycosyl linkage analyses, PLME-CP-III-1 was revealed as a novel galacturonic acid-rich acidic polysaccharide and further shown to play an important role in facilitating PP-mediated intestinal immunostimulatory activity. This is the first study demonstrating the structural characteristics of a novel intestinal immune system modulating acidic polysaccharide from P. linteus mycelium-containing whole culture broth postbiotics. © 2023 Elsevier B.V.
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