Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitisopen accessSaposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis
- Authors
- Erdenebileg, Saruul; Son, Yang-Ju; Kim, Myungsuk; Oidovsambuu, Sarangerel; Cha, Kwang Hyun; Kwon, Jaeyoung; Jung, Da Seul; Nho, Chu Won
- Issue Date
- Dec-2023
- Publisher
- Korea Institute of Oriental Medicine
- Keywords
- DSS-induced colitis; Gut microbiota; Inflammatory bowel disease; Inflammatory response; Saposhnikovia divaricata
- Citation
- Integrative Medicine Research, v.12, no.4, pp 1 - 12
- Pages
- 12
- Journal Title
- Integrative Medicine Research
- Volume
- 12
- Number
- 4
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/70462
- DOI
- 10.1016/j.imr.2023.100998
- ISSN
- 2213-4220
2213-4239
- Abstract
- Background: The root of Saposhnikovia divaricata (Turcz.) Schischk is a well-known traditional medicinal plant, containing various bioactive compounds with anti-inflammatory, antioxidant, and analgesic properties. However, no scientific studies have validated its clinical use as an anti-inflammatory agent against inflammatory bowel disease (IBD). This study aimed to investigate whether the root extract of S. divaricata ameliorates IBD and induces gut microbial alteration, using a RAW 264.7 cell line and a DSS-induced colitis mouse model. Methods: To investigate the anti-inflammatory effects and alleviation of IBD, using a methanol extract of Saposhnikovia divaricata (Turcz.) Schischk. root (MESD), RAW 264.7, murine macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model were employed. 16S rRNA gene sequencing was conducted to determine the alterations in the gut microbiota of mice with DSS-induced colitis. Results: MESD significantly decreased nitric oxide (NO) and inflammatory cytokine levels in lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro. Oral administration of MESD reduced the expression of inflammatory cytokines in the colons of mice with DSS-induced colitis. Additionally, MESD inhibited the abundance of Clostridium sensu stricto 1 and enhanced the predicted functional pathways, including L-glutamate degradation VIII (to propanoic acid). Seven compounds with anti-inflammatory properties were isolated from the MESD. Among them, 3′-O-acetylhamaudol and 3′-O-angeloylhamaudol exhibited strong anti-inflammatory effects in vitro. Conclusion: Overall, MESD may be a potential natural product for the treatment of IBD by lowering inflammatory cytokine levels and altering gut microbiota composition. © 2023
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