Description of Defluviimonas salinarum sp. nov. with the potential of benzene-degradation isolated from saltern in the Yellow Seacoast

Abstract

A novel species of Defluviimonas was isolated from a coastal saltern near the Yellow Sea. Strain CAU 1641(T) was isolated from saltern collected in Ganghwa Island, Republic of Korea. The bacterium was an aerobic, Gram-negative, catalase-positive, oxidase-positive, motile, and rod-shaped bacterium. Cell of strain CAU 1641(T) could grow at 20-40 degrees C and pH 6.0-9.0 with 1.0-3.0% (w/v) NaCl. Stain CAU 1641(T) shared high 16S rRNA gene sequence similarities with Defluviimonas aquaemixtae KCTC 42108(T) (98.0%), Defluviimonas denitrificans DSM 18921(T) (97.6%), and Defluviimonas aestuarii KACC 16442(T) (97.5%). Phylogenetic trees based on the 16S rRNA gene and the core-genome sequences indicated that strain CAU 1641(T) belonged to genus Defluviimonas. Strain CAU 1641(T) contained ubiquinone-10 (Q-10) as the sole respiratory quinone and and summed feature 8 (C-18:1 omega 6c and/or C-18:1 omega 7c) as the predominant fatty acid (86.1%). The pan-genome analysis indicated that the genomes of the strain CAU 1641(T) and 15 reference strains contain a small core genome. The Average Nucleotide Identity and digital DNA-DNA hybridization values among strain CAU 1641(T) and reference strains of the genus Defluviimonas were in the range of 77.6%-78.8% and 21.1-22.1%, respectively. The genome of strain CAU 1641(T) has several genes of benzene degradation. The genomic G + C content was 66.6%. Based on polyphasic and genomic analyses, strain CAU 1641(T) represents a novel species of the genus Defluviimonas, for which the name Defluviimonas salinarum sp. nov., is proposed. The type strain is CAU 1641(T) ( = KCTC 92081(T) = MCCC 1K07180(T)).