C-Glycoside-Metabolizing Human Gut Bacterium, Dorea sp. MRG-IFC3
- Authors
- Mi Huynh Thi Ngoc; Chaiyasarn Santipap; Kim Heji; Han Jaehong
- Issue Date
- Dec-2023
- Publisher
- 한국미생물·생명공학회
- Keywords
- C-C bond cleavage; C-glycoside; Dorea sp. MRG-IFC3; gut metabolism; puerarin
- Citation
- Journal of Microbiology and Biotechnology, v.33, no.12, pp 1606 - 1614
- Pages
- 9
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 33
- Number
- 12
- Start Page
- 1606
- End Page
- 1614
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71911
- DOI
- 10.4014/jmb.2308.08021
- ISSN
- 1017-7825
1738-8872
- Abstract
- Biochemical gut metabolism of dietary bioactive compounds is of great significance in elucidating health-related issues at the molecular level. In this study, a human gut bacterium cleaving C-C glycosidic bond was screened from puerarin conversion to daidzein, and a new, gram-positive Cglycoside-deglycosylating strain, Dorea sp. MRG-IFC3, was isolated from human fecal sample under anaerobic conditions. Though MRG-IFC3 biotransformed isoflavone C-glycoside, it could not metabolize other C-glycosides, such as vitexin, bergenin, and aloin. As evident from the production of the corresponding aglycons from various 7-O-glucosides, MRG-IFC3 strain also showed 7-Oglycoside cleavage activity; however, flavone 3-O-glucoside icariside II was not metabolized. In addition, for mechanism study, C-glycosyl bond cleavage of puerarin by MRG-IFC3 strain was performed in D2O GAM medium. The complete deuterium enrichment on C-8 position of daidzein was confirmed by 1 H NMR spectroscopy, and the result clearly proved for the first time that daidzein is produced from puerarin. Two possible reaction intermediates, the quinoids and 8-dehydrodaidzein anion, were proposed for the production of daidzein-8d. These results will provide the basis for the mechanism study of stable C-glycosidic bond cleavage at the molecular level.
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